recombinant human connective tissue growth factor (ctgf  (ProSpec)

Journal: Cell reports

Article Title: Directed differentiation of human iPSCs into mesenchymal lineages by optogenetic control of TGF-β signaling

doi: 10.1016/j.celrep.2023.112509

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Basal tenogenic medium was supplemented with additional factors: 50 μg/mL ascorbic acid 2-phosphate (Sigma), 100 ng/mL human recombinant growth/differentiation factor-7 (GDF-7; Peprotech), and after 3 days, 100 ng/mL human recombinant connective tissue growth factor (CTGF; Peprotech).

Techniques: Affinity Purification, Virus, Recombinant, Protease Inhibitor, SYBR Green Assay, Solvent, Plasmid Preparation, Control, Functional Assay, Bicinchoninic Acid Protein Assay, Reverse Transcription, Quantitative Proteomics, Software

Journal: Frontiers in Pharmacology

Article Title: Kinsenoside Protects Against Radiation-Induced Liver Fibrosis via Downregulating Connective Tissue Growth Factor Through TGF- β 1 Signaling

doi: 10.3389/fphar.2022.808576

Figure Lengend Snippet: The key fibrosis-related gene and signaling pathway that are inhibited by KD (A) The results of high-throughput sequencing of the transcriptome showed that the expression of CTGF gene increased after irradiation (B) The results of high-throughput sequencing of the transcriptome showed that the expression of CTGF gene decreased after the administration of KD (C) The results of Real-Time PCR were consistent with the results of the sequencing. The expression of CTGF mRNA increased in the IR group, but decreased in the IR + KD group (D) The results of western blotting were consistent with the results of sequencing. The expression of CTGF protein increased in the IR group, but decreased in the IR + KD group (E) In the transcriptome KEGG gene pathway enrichment map, the TGF-β pathway was closely related to CTGF and RILF (F) The results of tissue TGF-β1 immunohistochemistry indicated that in the IR + KD group the expression of TGF-β1 in the liver tissue decreased compared with the IR group. For all results in this figure, original magnification, ×40 and ×100. Mean ± SEM. * p < 0.05, ** p < 0.01.

Article Snippet: The antibodies included: α -SMA (1:1,000; Servicebio Technology, Wuhan, China), fibronectin (FN), collagen I and connective tissue growth factor (CTGF) (1:1,000; BOSTER Biological Technology, Wuhan, China), TGF- β 1 and Smad2/3 and P-Smad2/3 (1:1,000; Cell Signaling Technology Inc., Danvers, MA), GAPDH and β -actin (1:1,000; Aspen Biological, Wuhan, China).

Techniques: Next-Generation Sequencing, Expressing, Irradiation, Real-time Polymerase Chain Reaction, Sequencing, Western Blot, Immunohistochemistry

Journal: Frontiers in Pharmacology

Article Title: Kinsenoside Protects Against Radiation-Induced Liver Fibrosis via Downregulating Connective Tissue Growth Factor Through TGF- β 1 Signaling

doi: 10.3389/fphar.2022.808576

Figure Lengend Snippet: KD ameliorates the RILF by inhibiting TGF-β1/Smad/CTGF pathway (A) Exogenous TGF-β1 led to the increased expression of CTGF protein (B) Exogenous TGF-β1 induced an increase in the expression of collagen and a -SMA proteins (C) The TGF-β1 activity in the conditioned media significantly increased after 6Gy irradiation compared with the control group, while the TGF-β1 activity in the conditioned media in the IR + KD group decreased compared with the IR group (D-G) The expressions of TGF-β1 and downstream Smad2/3 as well as phosphorylated Smad2/3 reduced in the IR + KD group compared with the IR group. Mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The antibodies included: α -SMA (1:1,000; Servicebio Technology, Wuhan, China), fibronectin (FN), collagen I and connective tissue growth factor (CTGF) (1:1,000; BOSTER Biological Technology, Wuhan, China), TGF- β 1 and Smad2/3 and P-Smad2/3 (1:1,000; Cell Signaling Technology Inc., Danvers, MA), GAPDH and β -actin (1:1,000; Aspen Biological, Wuhan, China).

Techniques: Expressing, Activity Assay, Irradiation, Control

Journal: Frontiers in Pharmacology

Article Title: Kinsenoside Protects Against Radiation-Induced Liver Fibrosis via Downregulating Connective Tissue Growth Factor Through TGF- β 1 Signaling

doi: 10.3389/fphar.2022.808576

Figure Lengend Snippet: The schematic diagram. KD ameliorated the RILF through the regulation of the key molecule CTGF and TGF-β1/Smad/CTGF pathway in the HSCs.

Article Snippet: The antibodies included: α -SMA (1:1,000; Servicebio Technology, Wuhan, China), fibronectin (FN), collagen I and connective tissue growth factor (CTGF) (1:1,000; BOSTER Biological Technology, Wuhan, China), TGF- β 1 and Smad2/3 and P-Smad2/3 (1:1,000; Cell Signaling Technology Inc., Danvers, MA), GAPDH and β -actin (1:1,000; Aspen Biological, Wuhan, China).

Techniques:

Journal: Brazilian Journal of Pharmaceutical Sciences

Article Title: Swertiamarin attenuates carbon tetrachloride (CCl4)-induced liver injury and inflammation in rats by regulating the TLR4 signaling pathway

doi: 10.1590/s2175-97902018000417449

Figure Lengend Snippet: FIGURE 4 - STM repressed the protein expression of CTGF, TLR4 and NF-κB p65 in the liver. The CTGF protein level of rats in CCl4+STM 100 mg/kg and CCl4+STM 200 mg/kg groups were downregulated significantly compared with the CCl4 group. The protein expression of TLR4 and NF-κB p65 of rats in CCl4+STM 100 mg/kg and CCl4+STM 200 mg/kg groups were significantly lower than that in the CCl4 group. Data are represented as means ± S.D for 3-4 animals per group. * p < 0.05 versus control, ** p < 0.01 versus control; # p < 0.05 versus CCl4, ## p < 0.01 versus CCl4 by one-way ANOVA and LSD post hoc test.

Article Snippet: Antibodies against connective tissue growth factor (CTGF) (Boster, PB0570), TLR4 (Boster, BA1717), NFκB p65 (Santa Cruz, sc-1173), ser307pIRS-1 (Boster, P00268), GAPDH (Boster, BA2913), and Histone H3 (Santa Cruz, sc-166574) were used in this study.

Techniques: Expressing, Control

Journal: Stem Cells Translational Medicine

Article Title: Connective Tissue Growth Factor Promotes Efficient Generation of Human Induced Pluripotent Stem Cell‐Derived Choroidal Endothelium

doi: 10.1002/sctm.16-0399

Figure Lengend Snippet: Identification of CEC differentiation candidates using the Taguchi L12 and factorial elimination screens. (A) : Representative image used to determine the percentage of CA4 + iPSC‐derived CECs. (B) : Taguchi L12 half effect of each factor on the mean of percentage of CA4 + cells. (C) : Taguchi L12 half effect of each factor on the standard deviation of percentage of CA4 + cells. (D) : Mean (+SD) percentage of CA4 + cells elicited by each medium in the factorial elimination screen ( n ≥ 8, ****, p < .0001). (E–J) : Immunohistochemistry showing CTGF (E, G, green) and TWEAKR (H, J, green) expression in adult choroids. Sections were dual labeled with the endothelial cell binding lectin UEA‐I (F, G, I, J, red). All scale bars represent 100 μm. Abbreviations: CC, choriocapillaris; CEC, choroidal endothelial cell; CH, choroidal vasculature; CTGF, connective tissue growth factor; DAPI, 4′,6‐diamidino‐2‐phenylindole; RPE, retinal pigment epithelium; TWEAKR, TNF‐related weak inducer of apoptosis receptor; VEGFB, Vascular endothelial growth factor B.

Article Snippet: In order to perform the Taguchi L12 screen, we obtained recombinant human connective tissue growth factor (CTGF; Peprotech; Cat. No. 120‐19, https://www.peprotech.com/en-US ), human β‐catenin (CTNNB1; Sigma; Cat. No. SRP5172‐50UG), human Src Homology 2 Domain‐Containing Transforming Protein 1 (SHC1; Fitzgerald Industries; Cat. No. H00007453‐P01, https://www.fitzgerald-fii.com/ ), human TNF‐related weak inducer of apoptosis (TWEAK) receptor (TWEAKR also known as TNFRSF12A; PeproTech; Cat. No. 310‐21), and human vascular endothelial growth factor B (VEGFB; PeproTech; Cat. No. 100‐20B) proteins to use for the differentiation factor screening.

Techniques: Derivative Assay, Standard Deviation, Immunohistochemistry, Expressing, Labeling, Binding Assay

Journal: Stem Cells Translational Medicine

Article Title: Connective Tissue Growth Factor Promotes Efficient Generation of Human Induced Pluripotent Stem Cell‐Derived Choroidal Endothelium

doi: 10.1002/sctm.16-0399

Figure Lengend Snippet: CTGF drives iPSC differentiation into CECs. (A) : Mean (±SD; n = 9) percentage of CA4 + cells at varying concentrations of CTGF and TWEAKR. (B–E) : Representative images are provided showing cell morphology and CA4 expression at 0 ng/ml TWEAKR and 0 ng/ml CTGF (B), 0 ng/ml TWEAKR and 50 ng/ml CTGF (C), 10 ng/ml TWEAKR and 0 ng/ml CTGF (D), and 10 ng/ml TWEAKR and 50 ng/ml CTGF (E). (F) : Concentration of endogenously secreted CTGF in culture medium from human iPSCs differentiated into CEC in medium containing 10 ng/ml of TWEAKR alone or with varying concentrations of the inhibitor LY2109761, compared to spontaneously differentiated iPSCs grown in basal medium (control), as detected using ELISA. Shown are the means at each condition, while error bars represent the standard deviation ( n = 2). (G) : Percentage of CA4 + cells in cultures of human iPSCs differentiated using 10 ng/ml of TWEAKR alone or with 5 µM inhibitor (LY2109761), compared to spontaneously differentiated iPSCs grown in basal medium (control). Shown are the means at each condition, while error bars represent the standard error of the mean ( n = 9, **, p < .01, ***, p < .001). All scale bars represent 100 μm. Abbreviations: CEC, choroidal endothelial cell; CTGF, Connective tissue growth factor; DAPI, 4′,6‐diamidino‐2‐phenylindole; TWEAKR, TNF‐related weak inducer of apoptosis receptor.

Article Snippet: In order to perform the Taguchi L12 screen, we obtained recombinant human connective tissue growth factor (CTGF; Peprotech; Cat. No. 120‐19, https://www.peprotech.com/en-US ), human β‐catenin (CTNNB1; Sigma; Cat. No. SRP5172‐50UG), human Src Homology 2 Domain‐Containing Transforming Protein 1 (SHC1; Fitzgerald Industries; Cat. No. H00007453‐P01, https://www.fitzgerald-fii.com/ ), human TNF‐related weak inducer of apoptosis (TWEAK) receptor (TWEAKR also known as TNFRSF12A; PeproTech; Cat. No. 310‐21), and human vascular endothelial growth factor B (VEGFB; PeproTech; Cat. No. 100‐20B) proteins to use for the differentiation factor screening.

Techniques: Expressing, Concentration Assay, Control, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Stem Cells Translational Medicine

Article Title: Connective Tissue Growth Factor Promotes Efficient Generation of Human Induced Pluripotent Stem Cell‐Derived Choroidal Endothelium

doi: 10.1002/sctm.16-0399

Figure Lengend Snippet: Presence (+) or absence (−) of factors in media used for Taguchi L12 test conditions

Article Snippet: In order to perform the Taguchi L12 screen, we obtained recombinant human connective tissue growth factor (CTGF; Peprotech; Cat. No. 120‐19, https://www.peprotech.com/en-US ), human β‐catenin (CTNNB1; Sigma; Cat. No. SRP5172‐50UG), human Src Homology 2 Domain‐Containing Transforming Protein 1 (SHC1; Fitzgerald Industries; Cat. No. H00007453‐P01, https://www.fitzgerald-fii.com/ ), human TNF‐related weak inducer of apoptosis (TWEAK) receptor (TWEAKR also known as TNFRSF12A; PeproTech; Cat. No. 310‐21), and human vascular endothelial growth factor B (VEGFB; PeproTech; Cat. No. 100‐20B) proteins to use for the differentiation factor screening.

Techniques:

Journal: Stem Cells Translational Medicine

Article Title: Connective Tissue Growth Factor Promotes Efficient Generation of Human Induced Pluripotent Stem Cell‐Derived Choroidal Endothelium

doi: 10.1002/sctm.16-0399

Figure Lengend Snippet: Presence (+) or absence (−) of factors in media used for factor exclusion test conditions

Article Snippet: In order to perform the Taguchi L12 screen, we obtained recombinant human connective tissue growth factor (CTGF; Peprotech; Cat. No. 120‐19, https://www.peprotech.com/en-US ), human β‐catenin (CTNNB1; Sigma; Cat. No. SRP5172‐50UG), human Src Homology 2 Domain‐Containing Transforming Protein 1 (SHC1; Fitzgerald Industries; Cat. No. H00007453‐P01, https://www.fitzgerald-fii.com/ ), human TNF‐related weak inducer of apoptosis (TWEAK) receptor (TWEAKR also known as TNFRSF12A; PeproTech; Cat. No. 310‐21), and human vascular endothelial growth factor B (VEGFB; PeproTech; Cat. No. 100‐20B) proteins to use for the differentiation factor screening.

Techniques:

Journal: Stem Cells Translational Medicine

Article Title: Connective Tissue Growth Factor Promotes Efficient Generation of Human Induced Pluripotent Stem Cell‐Derived Choroidal Endothelium

doi: 10.1002/sctm.16-0399

Figure Lengend Snippet: RF/6A‐secreted factors of interest

Article Snippet: In order to perform the Taguchi L12 screen, we obtained recombinant human connective tissue growth factor (CTGF; Peprotech; Cat. No. 120‐19, https://www.peprotech.com/en-US ), human β‐catenin (CTNNB1; Sigma; Cat. No. SRP5172‐50UG), human Src Homology 2 Domain‐Containing Transforming Protein 1 (SHC1; Fitzgerald Industries; Cat. No. H00007453‐P01, https://www.fitzgerald-fii.com/ ), human TNF‐related weak inducer of apoptosis (TWEAK) receptor (TWEAKR also known as TNFRSF12A; PeproTech; Cat. No. 310‐21), and human vascular endothelial growth factor B (VEGFB; PeproTech; Cat. No. 100‐20B) proteins to use for the differentiation factor screening.

Techniques: